tlr 4 inhibitors  (InvivoGen)

Journal: iScience

Article Title: Deciphering the role of endothelial granulocyte macrophage-CSF in chronic inflammation associated with HIV

doi: 10.1016/j.isci.2024.110909

Figure Lengend Snippet: Single-cell RNA sequencing (RNAseq) reveals dynamic gene expression patterns in human aortic endothelial cells (ECs) ECs were cultured in medium alone or medium supplemented with LPS (100 ng/mL) or Dapa (1μg/mL) for 24 h. An additional 7days incubation period was included for estradiol (10 ng/mL) only (D5-E2-D7). (A). Unbiased clustering and UMAP representation of scRNAseq data analyzed with a resolution setting of 0.12, displaying distinct clusters of human aortic endothelial cells on a UMAP plot. (B) HeatMap of treatment-dependent differentially expressed genes illustrates the differential expression of genes in ECs across various treatment conditions, highlighting treatment-dependent clusters. (C and D) Violin plots display the expression profiles of selected genes (KLF2, eNOS, ICAM-1, VCAM-1, CSF-1, CSF2, and CSF3) within clusters from LPS treated cells. Expression levels are represented as probability distributions across clusters on the y axis.

Article Snippet: TLR-4 Inhibitor (TLR4-C34) (1μg/mL) , Cayman Chemical Co. , #18514.

Techniques: RNA Sequencing, Gene Expression, Cell Culture, Incubation, Quantitative Proteomics, Expressing

Journal: iScience

Article Title: Deciphering the role of endothelial granulocyte macrophage-CSF in chronic inflammation associated with HIV

doi: 10.1016/j.isci.2024.110909

Figure Lengend Snippet: Modulation of LPS-induced GM-CSF expression by human aortic endothelial cells (EC) through pharmacological interventions Endothelial cells were cultured in medium alone or medium supplemented with LPS (100 ng/mL), LPS + Dapa (1μg/mL) or LPS + TLR-4 Inhibitor (TLR4-C34) (10μg/mL). (A) Representative microscopy and fluorescent images of NBD glucose uptake under the indicated conditions (left) and violin plots show the quantification of NBD glucose uptake (MFI, right) in EC receiving indicated treatments (data from one of the 3 independent experiments, each circle represents MFI value from one EC, n = ∼100 cells/experiment, ∗∗∗ p < 0.0005). (B) Immunofluorescence microscopy images (left) and violin plots showing quantified image data (MFI/DAPI ratio, right) illustrating KLF2 protein expression in indicated treatment groups (data from 100 ECs per condition; each circle represents average MFI values from one image with 5–10 ECs). (C) ELISA data illustrate GM-CSF protein concentrations in supernatants of endothelial cell cultures after 24 h of incubation under specified conditions ( n = 3; ∗ p < 0.05). (D) Violin plots show ELISA data of GM-CSF levels in plasma samples from PWH, with ( n = 7) and without ( n = 10) receiving Dapa treatment (∗∗∗ p < 0.0005). All data are represented as mean ± SEM.

Article Snippet: TLR-4 Inhibitor (TLR4-C34) (1μg/mL) , Cayman Chemical Co. , #18514.

Techniques: Expressing, Cell Culture, Microscopy, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Incubation, Clinical Proteomics

Journal: iScience

Article Title: Deciphering the role of endothelial granulocyte macrophage-CSF in chronic inflammation associated with HIV

doi: 10.1016/j.isci.2024.110909

Figure Lengend Snippet:

Article Snippet: TLR-4 Inhibitor (TLR4-C34) (1μg/mL) , Cayman Chemical Co. , #18514.

Techniques: Virus, Microarray, Clinical Proteomics, Recombinant, Enzyme-linked Immunosorbent Assay, Isolation, Fluorescence, Plasmid Preparation, Gene Expression, Software